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5′-fluoro(di)phosphate-labeled oligonucleotides are versatile molecular probes for studying nucleic acid secondary structure and interactions by ¹⁹F NMR

Marek R Baranowski, Marcin Warminski, Jacek Jemielity, and Joanna Kowalska

This study describes the synthesis, characterization and application of oligonucleotides carrying 5′-fluoromonophosphate (FP) or 5′ fluorodiphosphate (FPP) moieties as ¹⁹F NMR probes of DNA structure and function. The authors synthesized several such labeled oligodeoxyribonucleotides using straightforward methods that avoid commercially unavailable phosphoramidite building blocks, and then validated their utility as molecular tools to: 1) probe duplex formation and distinguish 5′-terminal mismatches, 2) monitor G-quadruplex formation, 3) detect DNA-protein interactions and 4) monitor i-motif formation and its interactions with small molecule ligands.

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Reconstitution of the human tRNA splicing endonuclease complex: insight into the regulation of pre-tRNA cleavage

Cassandra K Hayne, Casey A Schmidt, Maira I Haque, A Gregory Matera, and Robin E Stanley

This study describes the biochemical reconstitution and characterization of the human tRNA Splicing Endonuclease ('TSEN') complex. This work is a major step forward in elucidating roles of the individual components of the TSEN machinery. It demonstrates that the complex is active in the presence and absence of the CLP1 kinase and confirms the identity of critical residues in the active sites of the endonuclease subunits, showing that the mechanism of tRNA cleavage is well conserved across archaeal and eukaryotic homologues. It further reveals that CLP1 is not required for TSEN mediated tRNA intron removal in vivo. Instead CLP1 functions as a negative regulator of tRNA exon ligation and intron circularization, providing insight into the role that CLP1 plays in tRNA splicing.

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Meet the Authors

Marek R Baranowski

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland
Dr Marcin Warminski

微拓客源助手 V1.0 安卓版 - 52z:2021-4-1 · 微拓客源助手，一款免费的微商加粉、互粉、拓展工具类app。软件介绍微拓客源助手ios版是郑州天点科技有限公司专门为苹果手机用户推出的一款手机加粉app，让广大的微商朋友再也不用为了没有客源烦恼了，快来下载体验吧！软件特色1.获取精准客户智能匹配精准
Dr Jacek Jemielity

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland
安卓微皮恩破解

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Warsaw, Poland
Dr Robin E Stanley

National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, USA
Prof. Xiaowo Wang

Ministry of Education Key Laboratory of Bioinformatics; Center for Synthetic and Systems Biology and Bioinformatics Division, Beijing National Research Center for Information Science and Technology; Department of Automation, Tsinghua University, Beijing, China
安卓微皮恩网盘

孩皮棋牌手机安卓版下载-孩皮棋牌正规手机版下载-72游戏网:2021-6-3 · 孩皮棋牌是一款体验感爆棚的联机对战中心。在软件内拥有轻松易上手的游戏规则众及最独特的棋牌玩法，好友之间还可众相互邀请对战，一键开局更加好玩。还在等什么，抓紧时间下载体验吧！
Ye Wang

Ministry of Education Key Laboratory of Bioinformatics; Center for Synthetic and Systems Biology and Bioinformatics Division, Beijing National Research Center for Information Science and Technology; Department of Automation, Tsinghua University, Beijing, China

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